![]() ![]() The Richter scale is logarithmic, meaning that for each increase in the magnitude there is a 10-fold increase in the shaking. The first part of the definition creates the most confusion. The definition is an excellent place to start dissecting the problems with the Richter magnitude. It is defined as the logarithm of the amplitude (measured in thousandths of millimeters or microns) of the largest seismic wave measured 100 km from the epicenter on a particular brand of seismometer. Finally, the fourth and perhaps least known is the best estimate of the size of the earthquake, the moment magnitude (M w) The Richter MagnitudeĬharles Richter developed the Richter magnitude in 1935. The body-wave (M B) and surface-wave magnitudes (M S) are offshoots of the Richter magnitude using those particular waves only. The most popular and well known is the Richter magnitude (M L), also known as the local magnitude. There are at least 4 different measurements of magnitude in use today. Earthquake magnitude: M B, M L, M S, M w - and what is the Richter Magnitude anyways? The size of earthquakes is typically given as a magnitude. ![]() When one asks "how big was that earthquake?" the answer is qualitative because it depends on what is meant by "big." There are several ways that the size of the earthquake can be measured quantitatively. How big was that quake? magnitudes, moments, and energy This quickly shows them that the magnitude of an earthquake is not the only determiner of damage and that anyone who uses magnitudes to talk about damage, clearly does not know much about earthquakes. one in Indonesia, one in Alaska, and one in Kamchatka! I then ask if they think the Needle would survive having one side of its supports moved 100 ft relative to the other side, a typical displacement on a fault producing a M9 quake. If you ride to the top of the Seattle Space Needle, much of the time the elevator operator will state that the needle was "built to withstand a magnitude 9 earthquake." I point out to my classes that the needle has withstood several M9 quakes without damage. Typically this will be shown by someone asking if a particular building will withstand a "magnitude 8" earthquake - and ignoring the distance, duration, hazards other than shaking, and other factors. People often think that magnitude of the earthquake is the only factor that determines damage. Earthquake shaking is typically measured as an acceleration higher magnitude earthquakes cause more violent shaking, which in turn typically cause higher intensity. There is one magnitude for an individual quake, but the intensity varies significantly. Earthquake magnitude is a measurement of the "size" of the quake - typically related to the amount of energy released. ![]() The difference between earthquake magnitude, intensity, and shakingĪsk many seismologists what the most critical and common misunderstanding about earthquakes is and they will answer "the difference between magnitude and intensity." Earthquake intensity and magnitude measure different things and are often misunderstood, and it is shaking that links them.Įarthquake intensity is a measurement of damage.
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8/13/2023 0 Comments Past tense er ir verbs spanish![]() ![]() The reason I said 1/2 a verb is because you only have to memorize the conjugations for the verb ver and combine those with the prefix. The other irregular verb that is like one of the main three but with a prefix is prever (to foresee). The main 3 irregular verbs in the Spanish past imperfect tense are ser (to be), ir (to go) and ver (to see). In Spanish, there are 3 irregular verbs in the past imperfect tense and one verb that is like one of these 3 but with a prefix. The past imperfect - irregular conjugationsįinally, you are up to the part where I explain what I mean by a 1/2 verb. I chose these 3 verbs at random but any verb in Spanish will follow this pattern except for the verbs in the next section. The conjugations for these three verbs are: Person So get excited!įirstly, the regular verb conjugations for the Spanish past imperfect come in two basic groups: one for ‘ ar‘ verbs and one for ‘ er‘ and ‘ ir‘ verbs.Īpart from the 3 and 1/2 verbs, you are going to learn in the next section, every Spanish verb is conjugated like the verbs pensar (to think), volver (to return) and sentir (to feel). In the next two sections, you are going to learn every conjugation in existence for the Spanish language in the past imperfect tense. The Spanish past imperfect - regular conjugations If you want to hear more about the use of the Spanish past imperfect check out the podcast episode. I know I’m getting a little philosophical here.īut, if you can grasp this argument, you will understand how and when to use the Spanish past imperfect tense. When did you first ‘not-know’ they were engaged?ĭid you start ‘not-knowing’ the moment your friend got engaged? Did you start ‘not-knowing’ when you were born because you couldn’t know stuff until you were born? Or does your ‘not-knowing’ extend right back to the start of time because nothing was known before then, right? But you couldn’t say that the ‘not-knowing’ had a start time. With this example, you could argue that the event of ‘not-knowing’ did have an end (right now). Here, the ‘not-knowing’ is the event, and it happened in the past because now in the present you do know. Imagine a good friend tells you that someone you know just got engaged. Here is an illustration that explains what I’m trying to say graphically. This type of scenario in the past signals that you need to use the Spanish past imperfect tense. ![]() In this second scenario, you can’t answer these questions because there isn’t a clearly defined start and end. When was the first glass drunk? When was the last? How many glasses have been consumed? There are tens or hundreds or even thousands of glasses of water that were drunk. Now with this statement, there isn’t a single event anymore or a defined start and end. “When we were young, my mother used to make us drink a glass of water with dinner every night.” The “event” was me drinking a glass of water and it had a clearly defined start and end. Then from that statement, you know at 1:01 PM the event hadn’t started yet, and at 1:03 PM the event had finished. “Yesterday, I drank a glass of water at 1:02 PM.” The first and most critical distinction you have to make to understand the Spanish past imperfect is that some “events” in the past don’t have a clearly defined start or end. How to use the Spanish past imperfect tense ![]() But first, let’s see where and how you should use the past imperfect tense. Later, I’ll explain what I mean by ½ a verb. And here’s a hint of what’s to come: there are only 3 and ½ irregular verbs for the whole language! I will also share with you what is awesome about irregular Spanish verbs in the past imperfect. In this post, you’ll learn how to use the Spanish past imperfect tense. There are, in fact, 7 past tenses in Spanish but I call the above tenses ‘the big 3’ because you only need to know these 3 tenses to be conversational in Spanish. The past imperfect tense is one of ‘the big 3’ Spanish past tenses including the present perfect and past preterite. This is particularly true when it comes to Spanish verbs in the past imperfect tense. You can reduce the workload by looking for patterns and common groups. But, there are ways to simplify the process. This makes learning irregular verbs a big task. ![]() ![]() ![]()
![]() ![]() It will enable you to keep track of the transfer efficiency of differently-sized proteins. Using a ladder in which each band is a different color can be beneficial. Instead, aim for a point at which most of the larger proteins have migrated to the membrane, and most small protein material is still on the membrane-not through it. Note that proteins of different molecular weights will migrate at different speeds-there is no magic moment at which all the proteins jump from the gel to the membrane. Your sample proteins on the SDS-PAGE gel will still be invisible, but you can check if the brightly colored ladder bands are still on the gel or have fully transferred to the membrane. Use a pre-stained protein ladder to track the transfer of proteins from a gel to a membrane. Use a Pre-Stained Molecular Weight Ladder 3 Tips to Optimize Your Western Blot Transfer 1. Instead, you will need to optimize the transfer time and conditions based on your equipment and the nature of your sample.īut how will you know when your transfer is complete? Well, this brings us to our transfer tips since these will enable you to optimize your Western blot transfers to achieve near-complete transfer. This means there is no easy way to determine how long you need to run the transfer to completely transfer all your proteins. For example, molecular weight and hydrophobicity influence protein retention. Transfer times are empirical and based on the properties of your samples. How Long Should I Transfer My Western Blot For? Because the apparatus for wet transfer is now relatively cheap, the capillary transfer method is somewhat archaic. ![]() Because of this, it’s more expensive than wet transfer.Ĭapillary transfer relies on diffusion to move your samples from the SDS-PAGE gel onto the blotting membrane instead of an electric current. The semi-dry/rapid transfer involves purchasing pre-wetted transfer sandwich components and using this with proprietary transfer devices such as the iBlot™. You manually soak the stack in a transfer buffer (hence wet) and assemble it yourself into a transfer sandwich. The wet transfer method is used in most laboratories and is probably the one you use. There are three main ways of transferring proteins in Western blotting: There’s already an excellent article on Bitesize Bio that discusses the different ways of transfer in Western blotting. What Are the Different Ways of Transfer in Western Blotting? ![]() Now let’s take a look at the different types of transfers in Western blotting. Fortunately, Bio-Rad has an article discussing transfer buffers, their composition, and compatibility. But be open to the idea that this buffer might not be suited to your samples.ĭesigning a tailor-made transfer buffer can get quite technical. You probably have a standard recipe for this somewhere in your lab. Transfer Bufferĭepending on the type of transfer you are performing, the transfer stack is usually wetted with a transfer buffer. While 0.45 µm is sufficient for most proteins, a smaller pore size might be necessary if you work with smaller proteins.īe sure to check out this article that explains the differences between the two membranes in detail. You also get a choice of pore sizes, so make sure to pick one suitable for your protein size. PVDF membranes can bind more protein than nitrocellulose membranes but may yield higher background. Usually, you have a choice between two transfer membranes: nitrocellulose membranes and polyvinylidene fluoride (PVDF) membranes.Ĭhoosing between them comes down to a choice between binding capacity vs. Two other key points are worth mentioning here. Going from top to bottom, the pieces are as follows: Wipe it down with damp tissue paper if you notice this is the case. When, for example, the transfer device becomes encrusted with buffer salts. This is because the stack itself can result in a poor transfer. Knowing the pieces of the stack will also make you a better scientist, which is never a bad thing! A simple transfer stack that is used for wet transfers. Check out the simple illustration in Figure 1 below. What Are the Parts of the Transfer Stack?īefore discussing ways to optimize your western blot transfer, it’s worth familiarizing yourself with the parts of the transfer stack. Here are three tips to optimize your Western blot transfers to enable you to get clear blots consistently. The cause can often be a poor transfer, which can easily be fixed by optimizing your western blot transfers. Does your western blot look like someone sat on black playdough? If you’re lamenting over yet another blotchy or bad western blot, don’t fret. ![]() 8/13/2023 0 Comments Dyn updater host status inactive![]() That valid HTTP header names conform to the regular expression Removed by the load balancer ( true), or routed to targets Indicates whether HTTP headers with header fields that are not valid are ![]() _mitigation_modeĭetermines how the load balancer handles requests that might pose a Prevent non-IGW internet access (such as, through peering, Transit Gateway,ĪWS Direct Connect, or AWS VPN). It is set to false for internet-facing load balancersĪnd true for internal load balancers. Unintended access to your internal load balancer through an internet ny_all_igw_trafficīlocks internet gateway (IGW) access to the load balancer, preventing Indicates whether deletion protection is enabled. ![]() The prefix for the location in the Amazon S3 bucket. For more information, see Enable access logs. The name of the Amazon S3 bucket for the access logs. Indicates whether access logs stored in Amazon S3 are enabled. Theįollowing table shows the size and EBS volume per instance type that the load balancer Integration, sticky sessions, authentication support, and integration withĪn Application Load Balancer can be deployed on c5/c5d, m5/m5d, or r5/r5d instances on an Outpost. The following features are not available: Lambda functions as targets, AWS WAF In the AWS Region for the Outpost, they are not used. You can register targets by instance ID or IP address. Reports an event to the AWS Health Dashboard and the If you do not have sufficient instanceĬapacity or available IP addresses to scale the load balancer, the load balancer You need additional capacity, the load balancer adds 4xlarge After scaling the nodes to the largest instance size, if The load balancer scales as needed, resizing the nodes one size at a timeĤxlarge). The supported instance types are shown in the following The load balancer requires two large instances on the Outpost for For more information, see the AWS Outposts User Guide. You must have a reliable network connection between your Outpost and itsĪWS Region. You must have installed and configured an Outpost in your on-premises dataĬenter. Timeouts when attempting to establish a connection. To serve traffic, but the stalled scaling attempt may cause 5xx errors or To scale, the Application Load Balancer will run with insufficient capacity. Note: If an Application Load Balancers subnet runs out of usable IP addresses while attempting Without them your Application Load Balancer could experienceĭifficulties with node replacement attempts, causing it to enter a failed state. Your load balancer uses these IP addresses toĮstablish connections with the targets. These eight IP addresses are required to allow the loadīalancer to scale out if needed. ![]() Least eight free IP addresses per subnet. To ensure that your load balancer can scale properly, verify that eachĪvailability Zone subnet for your load balancer has a CIDR block with at least a The following restrictionsĮach subnet must be from a different Availability Zone. You must select at least two Availability Zone subnets. ![]() 8/13/2023 0 Comments Free remote assistance windows 10![]() ![]() ![]() Modern solutions like JumpCloud Remote Assist have tightly integrated device management capabilities such as patch management, identity and access management, central logging, password management, and broad security insights across all digital assets. And these add-ons hinder a technician’s mobility and flexibility to work from anywhere or at any time. But often, these platforms require IT admins to relax firewall rules or complex VPN tunneling to prevent leaks or cyberattacks. Many remote assist solutions rely heavily on proprietary technology like Microsoft’s RDP or VNC-based solutions. Remote assistance is only as secure as the connection it has with the host computer - which can be compromised depending on where that person is working. When onboarding is tailored to an individual’s device, and IT has a full line of sight into technology issues, employees can get up to speed and back to work faster. And typically, remote assistance tools reduce the time IT spends diagnosing and fixing problems, giving them more time to work on other pressing tasks. It could also minimize field service work orders, reducing travel time and cost. ![]() With remote assistance, your IT team could be remote, eliminating the cost of office space. Sharing control lets IT see problems firsthand and streamlines communication, accelerating support and onboarding processes. Support and training can involve a lot of back and forth - especially when IT can’t fully see what’s happening behind the scenes. Combining a global IT team and remote assistance makes 24/7/365 support a reality. Their IT teams need to be able to solve problems and onboard employees across the world - even on mobile devices. Remote assistance can be used at any time from any location. They can easily diagnose what might be wrong with a device or guide someone through their computer setup without discussing it in person.īut there are other distinct benefits as well, including: Availability One obvious benefit of remote assistance is that IT can better serve remote workers. What Are the Benefits of Remote Assistance? Showing employees, vendors, or contractors how to do something is often more helpful than telling them how to do it. Other companies use remote assistance for training. Not only does this save the user time, but it also decreases the number of help work orders IT teams receive. Instead of sending over how-to guides, or screen sharing on tools like Zoom or Microsoft Teams, IT admins can take command of the user’s mouse and complete their device configuration in minutes. But when IT and MSPs can open files, photos, and videos, run scans, check registries, and access settings, they can easily verify a problem and brainstorm a fix in a much shorter time frame.īesides troubleshooting, remote assistance can also be used to onboard new remote employees. Often, users struggle to articulate problems over chat or video calls - and sending snapshots and files may not help. Remote assistance is particularly useful when remote employees have challenges with their devices or need to configure a brand-new one. Remote assistance is a connection between a user and technician that allows technicians and remote collaborators to view and operate the user’s device as if they were an admin. In this post, we’ll explain what remote assistance is, how it’s used, and the benefits you can expect from implementing it at remote-centric organizations. Remote assistance empowers IT admins and MSPs to connect to their end users’ devices anytime, anywhere, in real time. That’s where remote assistance tools come in handy. Many users find it tough to communicate what’s wrong or set up a new device without IT guiding them through the entire process step-by-step. Setting up new devices, training, the cost of password resets, and troubleshooting from afar can be extremely difficult - particularly without visibility into the user experience.Īnd while conferencing software can help, it’s often just a stopgap. The rise of remote offices has generated a lot of work for IT. ![]() 8/13/2023 0 Comments Fire hydrant nyc![]() ![]() It was fun, and I think I would go out looking for a dowsing no matter how my mother and father would give it to me afterwards. In the 1950s & 60s I was tossed in at every fire hydrant that was open on the Lower East Side. The Fire Department is not happy with that…it’s one of the reasons playground renovations include fountains that kids can play in. Opening a fire hydrant is a long city summer tradition (Ephemeral New York)
8/12/2023 0 Comments Europa 5 universalis![]() ![]() The Network for Greening the Financial System, which brings together 127 central banks and supervisors from all around the world, has played a crucial role in accelerating global action and will continue to do so. Third, central banks around the world can and must, within their mandates, support the greening of the financial system. More generally, we must identify and remove public and private barriers to green finance worldwide wherever possible. The review of the capital adequacy frameworks of multilateral development banks can offer such opportunity. The G20 – this year under India’s presidency – can play a key role in unlocking additional funding. Second, governments can push for reform of the multilateral financial architecture. Governments should also mobilise private finance by implementing transition policies and creating a sound and stable framework to attract capital flows at the national and global level. In taking up this challenge, there are at least three levers we can use to boost the funding needed for a green and just transition on a global scale.įirst and foremost, it is up to governments to lead the fight against climate change and honour their commitments to financing the transition.ĭeveloped economies must lead by example and honour the USD 100 billion climate pledge made 14 years ago at COP15 in Copenhagen. ![]() ECB research shows that in Europe alone, over 70% of our economy is highly dependent on nature’s ecosystem services – a figure that is likely to be much higher in developing economies. This means not only reducing greenhouse gas emissions to net zero and adapting our economies to shield them from climate change, but also tackling the root causes of the severe destruction of nature that is threatening the vital resources we rely on for our survival. The path forward is clear: we must forge ahead with a global transition to ensure our economies are future-proof. More than 90% of those who have perished owing to extreme weather events during the last half-century lived in these countries, where more than 70% of reported disasters occurred. One fact is glaringly evident: developing countries are poised to bear a disproportionate share of the impact. Developing nations are already the hardest hit by the impact of global warming. Not only is this a matter of justice for future generations, it is also undoubtedly a matter of justice and responsibility for today’s. It is everyone’s duty to take every possible step to ensure that the Paris climate goal is reached. ![]() They are inflicting suffering and damage on every continent and serve as a mere glimpse into the future. Record-breaking droughts, heatwaves and floods are already plaguing the world. Today, the window of opportunity to achieve this goal is closing before our eyes: The past eight years have been the warmest on record worldwide and the critical 1.5 C threshold for annual temperatures will likely be exceeded in at least one year before 2027. I am truly honoured to be with you here today at this important summit to address the most pressing issue of our time.Įight years ago in Paris, UN Secretary-General Ban Ki-moon opened the COP21 by stating that “Paris must mark a turning point ” towards limiting global warming to 1.5 ☌elsius. It has a small chance of appearing with the help of rebels during the campaign.Speech by Christine Lagarde, President of the ECB, at the Summit for a new global financing pact in Paris Revolter - This country lacks cores in 1444.It has a sizable chance of appearing with the help of rebels or being released in a peace deal during the campaign. Releasable - This country has cores in 1444, but is otherwise unplayable unless released by another country first.If it has been eliminated it may be formed by another country again. Formable - Other countries may form this country if it does not exist. ![]() The following table lists the dynamic tag types (first letter) and their max value. In terms of action order, dynamic tags act after the normal tags and then in the order of the table below. The tags are assigned according to order of creation. They use a different 3-key identifier structure: the first a letter (based on type) and the other two are numbers. įor example, if Sweden is moving an army into a province a Danish army is moving away from, and both their movement is set to resolve on the same day, Sweden will catch the army as they are listed prior to Denmark on the order list.ĭynamic tags are countries created during the campaign without a pre-existing tag. The tag order specifies in what order actions are resolved between tags. Tag order Īll countries in EU4 are identified by a tag. All existing nations at the beginning of the grand-campaign (). ![]() 8/12/2023 0 Comments We work remote![]() Claw hands Experts claim remote work has affected Anna’s hands. For every 20 minutes spent staring at a screen, work-from-homers should look away at something that is 20 feet away from them for 20 seconds, per Healthline. To mitigate this ocular side effect, remote workers should employ the 20-20-20 rule. “Without enough natural light or the bright lights provided in commercial offices, remote workers will be putting extra strain on their eyes which could lead to headaches,” the site writes. Furniture at WorkĪlong with an apostrophe-like posture, Anna also suffers from bleary red eyes, the apparent result of the aforementioned awkward posture coupled with remote workers’ tendency to work sans proper lighting. Red eyes Anna’s eyes are affected by the lack of light. In September, a survey of UK workers found that 63.7% had newfound back issues since working from home. “Chronic pains and degenerative conditions can also develop because of prolonged sitting and inadequate posture (especially when no one is watching).” “Lack of regular movement and ergonomic inefficiencies can lead to musculoskeletal disorders,” said orthopedic surgeon Dr. This is the result of years of neck and back strain due to poor computer posture, or working from a sofa or a bed, per the site. Furniture at WorkĪs the model shows, one of the main side effects is developing a hunched back like a reverse-evolutionary Progression of Man chart. They then enlisted a designer to concoct a 3D model of someone whose body has evolved due to the consistent use of “laptops and smartphones, poor posture and an unhealthy diet.” Back at home Anna suffers from poor posture due to years of remote work. To generate Anna, sourced scientific research and collaborated with health experts to examine the “potentially damaging health effects of working from home.” Meanwhile, 28% worked on a hybrid schedule. Pew Research Center found that as much as 14% of the US population is working from home all the time. ![]() This model was concocted as work-from-home rates continue to soar since the pandemic. Anna, a model developed by office furniture company represents what remote workers will look like in the year 2100. ![]() “Anna displays many physical effects because of consistent use of technology, screen exposure and poor posture, as well as highlighting potential mental health issues,” described in the study, which coincidentally comes as companies are increasingly demanding that workers return to the office. However, researchers claim the human race could suffer a frightening devolution should we continue working from home - which apparently includes sprouting gnarled troll hands and a hunchback in under 100 years.Ĭase in point: Anna, a 3D model created by UK furniture company which is supposed to represent what we will look like by the year 2100, should we prolong this arrangement. Work from home, empty offices leading to ‘doom loop’ for NYC: studyįarmers Insurance workers blast return-to-office mandate: ‘I moved closer to grandkids’įirst becoming in vogue during the COVID-19 pandemic, remote work continues to be the modus operandi for many employees today. Empty offices push restaurant owners to pivot to NYC’s work-from-home hotspotsġ in 7 remote staffers have sex, shop, nap instead of working full day: survey ![]() 8/12/2023 0 Comments Awa artist alley application![]() (These are picked up on set up day and are not mailed out, additional badges may be purchased for $80 each on site) Two (2) Exhibitor badges per 6x5 Dealer Table space.No pipe and drape: you may bring your own display setup, however, it must remain within your allotted space.ĭealer Tables (6x5 spaces) | These are for anyone pop culture related that is looking for less space than a 10x10 booth.Internet is not included but available through the Salt Palace Convention Center for an additional fee.Two (2) Exhibitor badges per 6x5 Artist Alley Table space.*you are not able to purchase a table for a friend or gift them one of your tables *Artist Alley is for 2-D artwork, 3-D Artwork, handmade items made by YOURSELF, original comic, original book and original illustrated book only. Internet is not included but available through the Salt Palace Convention Center for an additional fee.Electrical is not included but available through Modern Display for an additional fee.Four (4) Exhibitor badges per 10x10 space. ![]() One ID sign (if purchased prior to 30 days from event).One six foot skirted table (Regardless of corner or inline space).Helium Balloons are not permitted at any location You can't create your own layout is what I am getting at! For example: A purchase of one inline line item and one corner line item is not valid and will be refunded. You may only purchase ONE of the pre-made layouts that are available (do not combine them) unless you are looking for multiple locations, then you must make a separate purchase order. Information September 22-24, 2022| Salt Lake City, UT | Salt Palace Convention Center Exhibitor Details Walkway easement directly behind your space (Can not be used for storage as this is an access point to and from everyone's space!).Power and Wifi are NOT included but will be available for purchase through the show decorator and facility.Pipe and drape walls with one 8' high back and two sides - either 3' or 8' high depending upon location and configuration. ![]()
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